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It's useful to assess the retention time distribution as a quality control step. The peptides should be spread across the retention times, without any clear 'gaps' or very clear trend towards one end of the gradient.

Usage

plot_rt_dist(obj, rt_col = "RT.in.min.by.Search.Engine.Sequest.HT")

Arguments

obj

SummarisedExperiment containing peptide-level output from Proteome Discoverer.

rt_col

string. Name of column with retention time

Value

Returns a ggplot with the RT vs delta PPM